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<blockquote style="position: relative; padding-left: 55px;"><section><a href="https://mstdn.science/users/STCmicrobeblog/statuses/113559969284018053">Small Things Considered (stcmicrobeblog@mstdn.science)'s status on Thursday, 28-Nov-2024 19:36:15 JST</a><a href="https://mstdn.science/@STCmicrobeblog" title="stcmicrobeblog@mstdn.science"><img src="https://gnusocial.jp/avatar/294873-48-20241112082146.webp" width="48" height="48" alt="Small Things Considered" style="position: absolute; left: 0; top: 0;">Small Things Considered</a><div><a href="https://qoto.org/@freemo/113559048051166022" rel="in-reply-to">in reply to</a><ul><li></ul></div></section><article><p><a href="https://qoto.org/@freemo">@freemo</a> </p><p>"My skepticism about the authors' claim that the genome of 𝘛. 𝘮𝘢𝘨𝘯𝘪𝘧𝘪𝘤𝘢 is localized in membrane-bound compartments ─ which they call "pepins" ─ stems from the fact that their technically excellent EM images do not show a continuous membrane, at least not in one focal plane (Figure 4, F+G)."<br>.<br><a href="https://schaechter.asmblog.org/schaechter/2022/05/magnificently-long-thiomargarita-magnifica.html" rel="nofollow noreferrer">https://schaechter.asmblog.org/schaechter/2022/05/magnificently-long-thiomargarita-magnifica.html</a></p></article><footer><a rel="bookmark" href="https://gnusocial.jp/conversation/4084988#notice-7983413">In conversation</a><time datetime="2024-11-28T19:36:15+09:00" title="Thursday, 28-Nov-2024 19:36:15 JST">about 3 months ago</time> <span>from <span><a href="https://mstdn.science/@STCmicrobeblog/113559969284018053" rel="external" title="Sent from mstdn.science via ActivityPub">mstdn.science</a></span></span><a href="https://mstdn.science/@STCmicrobeblog/113559969284018053">permalink</a><h4>Attachments</h4><ol><li><label><a rel="external" href="https://gnusocial.jp/attachment/3586352">Characterization of the pepin organelles by FISH and correlative TEM as well as membrane and DNA staining. A─D: Pepins (arrows) in the cytoplasm of "Ca. T. magnifica" (class Gammaproteobacteria) are labeled with the general bacterial probe EUB labeled with Alexa Fluor 488 (A, green), Gammaproteobacteria specific 5 probe Gam42a labeled with Cy3 (B, yellow), Thiomargarita-specific probe Thm482 labeled with Cy5 (C, red) and with DAPI (D, blue). E TEM of a serial thin section consecutive to the semi-thin section used for FISH. The FISH and DAPI positive pepins appear as electron dense organelles under TEM. F,G Pepins (from E) under higher magnification; pepins are delimited by a membrane (arrowheads) and contain numerous ribosomes which appear as small electron dense granules. Source https://www.biorxiv.org/content/10.1101/2022.01.26.477692v1</a></label><br><a href="https://static.mstdn.science/media_attachments/files/113/559/959/552/928/691/original/7582fd9686c4f82a.png" rel="external">https://static.mstdn.science/media_attachments/files/113/559/959/552/928/691/original/7582fd9686c4f82a.png</a></li><li><label><a rel="external" href="https://gnusocial.jp/attachment/3586353">Untitled attachment</a></label><br></li></ol></footer></blockquote>

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Small Things Considered (stcmicrobeblog@mstdn.science)'s status on Thursday, 28-Nov-2024 19:36:15 JSTSmall Things Considered
in reply to
- 🎓 Doc Freemo :jpf: 🇳🇱
@freemo
"My skepticism about the authors' claim that the genome of 𝘛. 𝘮𝘢𝘨𝘯𝘪𝘧𝘪𝘤𝘢 is localized in membrane-bound compartments ─ which they call "pepins" ─ stems from the fact that their technically excellent EM images do not show a continuous membrane, at least not in one focal plane (Figure 4, F+G)."
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https://schaechter.asmblog.org/schaechter/2022/05/magnificently-long-thiomargarita-magnifica.html
In conversationabout 3 months ago from mstdn.sciencepermalink
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1. Characterization of the pepin organelles by FISH and correlative TEM as well as membrane and DNA staining. A─D: Pepins (arrows) in the cytoplasm of "Ca. T. magnifica" (class Gammaproteobacteria) are labeled with the general bacterial probe EUB labeled with Alexa Fluor 488 (A, green), Gammaproteobacteria specific 5 probe Gam42a labeled with Cy3 (B, yellow), Thiomargarita-specific probe Thm482 labeled with Cy5 (C, red) and with DAPI (D, blue). E TEM of a serial thin section consecutive to the semi-thin section used for FISH. The FISH and DAPI positive pepins appear as electron dense organelles under TEM. F,G Pepins (from E) under higher magnification; pepins are delimited by a membrane (arrowheads) and contain numerous ribosomes which appear as small electron dense granules. Source https://www.biorxiv.org/content/10.1101/2022.01.26.477692v1
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