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Characterization of the pepin organelles by FISH and correlative TEM as well as membrane and DNA staining. A─D: Pepins (arrows) in the cytoplasm of "Ca. T. magnifica" (class Gammaproteobacteria) are labeled with the general bacterial probe EUB labeled with Alexa Fluor 488 (A, green), Gammaproteobacteria specific 5 probe Gam42a labeled with Cy3 (B, yellow), Thiomargarita-specific probe Thm482 labeled with Cy5 (C, red) and with DAPI (D, blue). E TEM of a serial thin section consecutive to the semi-thin section used for FISH. The FISH and DAPI positive pepins appear as electron dense organelles under TEM. F,G Pepins (from E) under higher magnification; pepins are delimited by a membrane (arrowheads) and contain numerous ribosomes which appear as small electron dense granules. Source https://www.biorxiv.org/content/10.1101/2022.01.26.477692v1

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Characterization of the pepin organelles by FISH and correlative TEM as well as membrane and DNA staining. A─D: Pepins (arrows) in the cytoplasm of "Ca. T. magnifica" (class Gammaproteobacteria) are labeled with the general bacterial probe EUB labeled with Alexa Fluor 488 (A, green), Gammaproteobacteria specific 5 probe Gam42a labeled with Cy3 (B, yellow), Thiomargarita-specific probe Thm482 labeled with Cy5 (C, red) and with DAPI (D, blue). E TEM of a serial thin section consecutive to the semi-thin section used for FISH. The FISH and DAPI positive pepins appear as electron dense organelles under TEM. F,G Pepins (from E) under higher magnification; pepins are delimited by a membrane (arrowheads) and contain numerous ribosomes which appear as small electron dense granules. Source https://www.biorxiv.org/content/10.1101/2022.01.26.477692v1
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Small Things Considered (stcmicrobeblog@mstdn.science)'s status on Thursday, 28-Nov-2024 19:36:15 JST Small Things Considered
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@freemo
"My skepticism about the authors' claim that the genome of 𝘛. 𝘮𝘢𝘨𝘯𝘪𝘧𝘪𝘤𝘢 is localized in membrane-bound compartments ─ which they call "pepins" ─ stems from the fact that their technically excellent EM images do not show a continuous membrane, at least not in one focal plane (Figure 4, F+G)."
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https://schaechter.asmblog.org/schaechter/2022/05/magnificently-long-thiomargarita-magnifica.html

In conversation about 8 months ago from mstdn.science permalink